Matrix Metalloproteinases 2 & 9: Novel Biomarkers for Sepsis Severity?
Keywords:
MedicineAbstract
Sepsis is a common clinical syndrome and a significant cause of death worldwide. The development of thrombocytopenia is a determinant prognostic factor in these patients. Matrix metalloproteinase (MMP) -2 and -9 are released from platelets during aggregation, and leukocytes during inflammation respectively. Upregulation of MMP-9 in plasma of septic patients has been demonstrated but little is known regarding MMP-2 levels. We aimed to identify if MMP-2 activity in the plasma and platelet membranes of septic patients correlated with increasing disease severity.
A platelet poor plasma fraction and a platelet pellet fraction were separated by differential centrifugation from blood samples of healthy controls (n=9), septic non-thrombocytopaenic (n=6) and septic thrombocytopaenic (n=3) patients. Following protein concentration standardisation, MMP -2 and -9 activity in each of the two donor fractions was determined using gelatin zymography and quantified by densitometry. MMP-2 and MMP-9 activity was upregulated in the plasma of septic patients compared to healthy controls as was MMP-9 activity in the platelet pellet of septic patient samples compared to healthy controls. There was a trend whereby MMP-2 activity was further upregulated in the thrombocytopaenic patients.We conclude that plasma MMP-2 activity may pro-vide a novel non-specific marker for increasing severity of systemic inflammation.
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